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Proteome analysis as a non-invasive tool in transplant monit. By Dr W. Gwinner, Dr F. Rohde and Prof. H. Mischak


It is important that any post-transplantation complications, such as rejection, be identified as early as possible so that appropriate therapy can be initiated. Currently definite identification of such complications involves the analysis of biopsies, which is not suitable for repetitive

monitoring. Studies of the levels of many individual proteins found in more accessible body fluids have been undertaken to determine their utility as early biomarkers, but no single marker has sufficient diagnostic sensitivity or specificity to be of practical use. Whole proteome analysis of body fluids, however, has the potential of identifying patterns of biomarkers. This article reviews the various techniques used in such proteome analyses. CE-MS seems the most promising of the techniques and has already enabled the identification of a specific peptide pattern that can distinguish rejection in kidney transplant patients. Proteome analysis as a non-invasive tool in transplant monit




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