The use of HPLC in routine diagnostics
The use of HPLC in routine diagnostics. The evolution of liquid chromatography to high
performance liquid chromatography (HPLC) at
the beginning of the nineteen seventies was characterised by much improved column separation capabilities. As a result, the separation of mixtures of substances with similar chemical and physical properties became possible. In the years which followed,
this new technology spread to research
facilities and universities. This led to further improvement to the HPLC technique in general (e.g. pumps, detectors, auto samplers) as well as different column packing materials. However these advances were not applicable to smaller laboratories until the beginning of the nineties, because of some of the complex, time-consuming, and sometimes high-risk procedures involved. In particular the analysis of endogenous and high molecular
weight substances could only be carried out by
technicians with considerable experience, and
therefore such analyses were normally carried out in large research-oriented laboratories. The relatively small numbers of tests requested for these analytes made other assay methods, such as immunoassays, which focussed on tests for more frequently requested analytes, inappropriate. In addition the troublesome and labour-intensive preparation of samples for HPLC made the method uneconomic.
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