ELISA for determination of oxidised DNA
ELISA for DNA
Normal oxygen metabolism produces reactive oxygen species (ROS), highly reactive molecules that can damage DNA, RNA, lipids and proteins. While cells are normally able to defend themselves against such damage with a range of anti-oxidants and efficient DNA repair mechanisms, increased levels of ROS lead to oxidative stress. In nuclear and mitochondrial DNA, the principal result of free radical-induced oxidation is the creation of 8-hydroxy-2’ -deoxyguanosine (8-OHdG) or 8-oxo-7,8-dihydro-2’ -deoxyguanosine (8-oxodG) moieties, which are therefore widely used as biomarkers for oxidative stress and carcinogenesis. Studies have shown that in particular urinary 8-OHdG is a good biomarker for the assessment of the risk of various cancers and degenerative diseases. The oxidized DNA ELISA test from Bühlmann allows a direct, quantitative determination of 8-OHdG in human urine which should be diluted 1:10 prior to the assay. The competitive ELISA test uses a proprietary, specific monoclonal antibody so there is no cross-reactivity (apart from oxidized RNA, 8-OHG, 20% and the oxidized base, 8-OHGua, 17%). The assay is therefore eminently suitable for the specific detection of the complete oxidative status of the genetic material of the organism. Supplied as a complete kit, including coated microtitre plate, calibrators and internal controls, the new test correlates well with the “New 8-OHdG Check” from the Japan Institute for the Control of Aging (JaICA) which was, up till now, considered to be the reference method.
BÜHLMANN Laboratories
Schönenbuch, Switzerland
Bühlmann Laboratories AG
